Selected recent publications:

2023

Parkinson's disease kinase LRRK2 coordinates a cell-intrinsic itaconate-dependent defence pathway against intracellular Salmonella. Lian H, Park D, Chen M, Schueder F, Lara-Tejero M, Liu J, Galán JE. Nat Microbiol . 2023 Aug 28. doi: 10.1038/s41564-023-01459-y.

The sorting platform in the type III secretion pathway: From assembly to function. Soto JE, Lara-Tejero M. Bioessays. 2023 Sep;45(9):e2300078. doi: 10.1002/bies.202300078.

2022

Assembly and architecture of the type III secretion sorting platform. Soto JE, Galán JE, Lara-Tejero M. Proc Natl Acad Sci U S A. 2022 Dec 20;119(51):e2218010119. doi: 10.1073/pnas.2218010119.

Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cells. Chang SJ, Hsu YT, Chen Y, Lin YY, Lara-Tejero M, Galan JE. Elife. 2022 May 17;11:e78561. doi: 10.7554/eLife.78561.

2021

Cryo-EM structure of the needle filament tip complex of the Salmonella type III secretion injectisome. Guo EZ, Galán JE. Proc Natl Acad Sci U S A. 2021 Nov 2;118(44):e2114552118. doi: 10.1073/pnas.2114552118.

Salmonella Typhimurium and inflammation: a pathogen-centric affair. Galán JE. Nat Rev Microbiol. 2021 Nov;19(11):716-725. doi: 10.1038/s41579-021-00561-4.

The Salmonella effector protein SopD targets Rab8 to positively and negatively modulate the inflammatory response. Lian H, Jiang K, Tong M, Chen Z, Liu X, Galán JE, Gao X. Nat Microbiol. 2021 May;6(5):658-671. doi: 10.1038/s41564-021-00866-3.

2020

Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella. Chen M, Sun H, Boot M, Shao L, Chang SJ, Wang W, Lam TT, Lara-Tejero M, Rego EH, Galán JE. Science. 2020 Jul 24;369(6502):450-455. doi: 10.1126/science.aaz1333.

Generation and Characterization of Typhoid Toxin-Neutralizing Human Monoclonal Antibodies. Jiao X, Smith S, Stack G, Liang Q, Bradley A, Kellam P, Galán JE. Infect Immun. 2020 Sep 18;88(10):e00292-20. doi: 10.1128/IAI.00292-20.

Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase. Geiger T, Lara-Tejero M, Xiong Y, Galán JE. Elife. 2020 Jan 20;9:e53473. doi: 10.7554/eLife.53473.

2019

High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway. Butan C, Lara-Tejero M, Li W, Liu J, Galán JE. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24786-24795. doi: 10.1073/pnas.1916331116. Epub 2019

Alternate subunit assembly diversifies the function of a bacterial toxin. Fowler CC, Stack G, Jiao X, Lara-Tejero M, Galán JE. Nat Commun. 2019 Aug 15;10(1):3684. doi: 10.1038/s41467-019-11592-0

A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion. Guo EZ, Desrosiers DC, Zalesak J, Tolchard J, Berbon M, Habenstein B, Marlovits T, Loquet A, Galán JE. PLoS Biol. 2019 Jul 1;17(7):e3000351. doi: 10.1371/journal.pbio.3000351. eCollection 2019 Jul.

Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen. Shu-Jung Chang, Sheng Chih Jin, Xuyao Jiao, Jorge E. Galán. PLoS Pathog. 2019 Apr 5;15(4):e1007704. doi: 10.1371/journal.ppat.1007704.

The Injectisome, a Complex Nanomachine for Protein Injection into Mammalian Cells. Maria Lara-Tejero, Jorge E. Galán. EcoSal Plus. 2019 Mar;8(2). doi: 10.1128/ecosalplus.ESP-0039-2018.

Role of SpaO in the assembly of the sorting platform of a Salmonella type III secretion system. Maria Lara-Tejero, Zhuan Qin, Bo Hu, Carmen Butan, Jun Liu, Jorge E. Galán. PLoS Pathog. 2019 Jan 22;15(1):e1007565. doi: 10.1371/journal.ppat.1007565.

2018

Peptidoglycan editing by a specific LD-transpeptidase controls the muramidase-dependent secretion of typhoid toxin. Tobias Geiger, Manuel Pazos, Maria Lara-Tejero, Waldemar Vollmer, Jorge E. Galán. Nat Microbiol. 2018 Nov;3(11):1243-1254. doi: 10.1038/s41564-018-0248-x.

Salmonella stimulates pro-inflammatory signalling through p21-activated kinases bypassing innate immune receptors. Hui Sun, Jana Kamanova , Maria Lara-Tejero, Jorge E. Galán. Nat Microbiol. 2018 Oct;3(10):1122-1130. doi: 10.1038/s41564-018-0246-z.

A protein secreted by the Salmonella type III secretion system controls needle filament assembly. Junya Kato, Supratim Dey, Jose E. Soto, Carmen Butan, Mason C. Wilkinson, Roberto N. De Guzman, Jorge E. Galán. Elife. 2018 Jul 17;7. pii: e35886. doi: 10.7554/eLife.35886.

Protein-Injection Machines in Bacteria. Jorge E. Galán, Gabriel Waksman. Cell. 2018 Mar 8;172(6):1306-1318. doi: 10.1016/j.cell.2018.01.034.

Decoding a Salmonella Typhi Regulatory Network that Controls Typhoid Toxin Expression within Human Cells. Casey C. Fowler, Jorge E. Galán. Cell Host Microbe. 2018 Jan 10;23(1):65-76.e6. doi: 10.1016/j.chom.2017.12.001.

2017

Evolution of host adaptation in the Salmonella typhoid toxin. Xiang Gao, Lingquan Deng, Gabrielle Stack, Hai Yu, Xi Chen, Yuko Naito-Matsui, Ajit Varki, Jorge E. Galán. Nat Microbiol. 2017 Dec;2(12):1592-1599. doi: 10.1038/s41564-017-0033-2.

Salmonella enterica serovar-specific transcriptional reprogramming of infected cells. Sebastian Hannemann, Jorge E. Galán. PLoS Pathog. 2017 Jul 24;13(7):e1006532. doi: 10.1371/journal.ppat.1006532.

Visualization and characterization of individual type III protein secretion machines in live bacteria. Yongdeng Zhang, María Lara-Tejero, Jörg Bewersdorf, and Jorge E. Galán. Proc Natl Acad Sci USA. 2017 Jun 6;114(23):6098-6103. doi: 10.1073/pnas.1705823114.

Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni. Beile Gao, Hanne Vorwerk, Claudia Huber, Maria Lara-Tejero, Juliane Mohr, Andrew L. Goodman, Wolfgang Eisenreich, Jorge E. Galán, Dirk Hofreuter. PLoS Biol. 2017 May 19;15(5):e2001390. doi: 10.1371/journal.pbio.2001390.

In Situ Molecular Architecture of the Salmonella Type III Secretion Machine. Bo Hu, Maria Lara-Tejero, Qingke Kong, Jorge E. Galán, Jun Liu. Cell. 2017 Mar 9;168(6):1065-1074.e10. doi: 10.1016/j.cell.2017.02.022.

Emerging insights into the biology of typhoid toxin. Casey C. Fowler, Shu-Jung Chang, Xiang Gao, Tobias Geiger, Gabrielle Stack, Jorge E. Galán. Curr Opin Microbiol. 2017 Feb;35:70-77. doi: 10.1016/j.mib.2017.01.012.

2016

Typhoid toxin provides a window into typhoid fever and the biology of Salmonella Typhi. Galán JE. Proc Natl Acad Sci U S A. 2016 Jun 7;113(23):6338-44. doi: 10.1073/pnas.1606335113.

The Salmonella Effector Protein SopA Modulates Innate Immune Responses by Targeting TRIM E3 Ligase Family Members. Kamanova J, Sun H, Lara-Tejero M, Galán JE. PLoS Pathog. 2016 Apr 8;12(4):e1005552. doi: 10.1371/journal.ppat.1005552.

A Family of Salmonella Type III Secretion Effector Proteins Selectively Targets the NF-κB Signaling Pathway to Preserve Host Homeostasis. Sun H, Kamanova J, Lara-Tejero M, Galán JE. PLoS Pathog. 2016 Mar 2;12(3):e1005484. doi: 10.1371/journal.ppat.1005484.

Absence of TLR11 in Mice Does Not Confer Susceptibility to Salmonella Typhi. Song J, Wilhelm CL, Wangdi T, Maira-Litran T, Lee SJ, Raetz M, Sturge CR, Mirpuri J, Pei J, Grishin NV, McSorley SJ, Gewirtz AT, Bäumler AJ, Pier GB, Galán JE, Yarovinsky F. Cell. 2016 Feb 25;164(5):827-8. doi: 10.1016/j.cell.2016.02.015.

Determination of the Stoichiometry of the Complete Bacterial Type III Secretion Needle Complex Using a Combined Quantitative Proteomic Approach. Zilkenat S, Franz-Wachtel M, Stierhof YD, Galán JE, Macek B, Wagner S. Mol Cell Proteomics. 2016 May;15(5):1598-609. doi: 10.1074/mcp.M115.056598.

A Bacterial Pathogen Targets a Host Rab-Family GTPase Defense Pathway with a GAP. Spanò S, Gao X, Hannemann S, Lara-Tejero M, Galán JE. Cell Host Microbe. 2016 Feb 10;19(2):216-26. doi: 10.1016/j.chom.2016.01.004.

Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates. Tsou LK, Lara-Tejero M, RoseFigura J, Zhang ZJ, Wang YC, Yount JS, Lefebre M, Dossa PD, Kato J, Guan F, Lam W, Cheng YC, Galán JE, Hang HC. J Am Chem Soc. 2016 Feb 24;138(7):2209-18. doi: 10.1021/jacs.5b11575.

2015

Role of autocleavage in the function of a type III secretion specificity switch protein in Salmonella enterica serovar Typhimurium. Monjarás Feria JV, Lefebre MD, Stierhof YD, Galán JE, Wagner S. MBio. 2015 Oct 13;6(5):e01459-15. doi: 10.1128/mBio.01459-15.

Structural Features Reminiscent of ATP-Driven Protein Translocases Are Essential for the Function of a Type III Secretion-Associated ATPase. Kato J, Lefebre M, Galán JE. J Bacteriol. 2015 Sep;197(18):3007-14. doi: 10.1128/JB.00434-15.

2014

Host adaptation of a bacterial toxin from the human pathogen Salmonella Typhi. Deng L, Song J, Gao X, Wang J, Yu H, Chen X, Varki N, Naito-Matsui Y, Galán JE, Varki A. Cell. 2014 Dec 4;159(6):1290-9. doi: 10.1016/j.cell.2014.10.057.

Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells. Galán JE, Lara-Tejero M, Marlovits TC, Wagner S. Annu Rev Microbiol. 2014;68:415-38. doi: 10.1146/annurev-micro-092412-155725.

Novel components of the flagellar system in epsilonproteobacteria. Gao B, Lara-Tejero M, Lefebre M, Goodman AL, Galán JE. MBio. 2014 Jun 24;5(3):e01349-14. doi: 10.1128/mBio.01349-14.

NMR model of PrgI-SipD interaction and its implications in the needle-tip assembly of the Salmonella type III secretion system. Rathinavelan T, Lara-Tejero M, Lefebre M, Chatterjee S, McShan AC, Guo DC, Tang C, Galan JE, De Guzman RN. J Mol Biol. 2014 Aug 12;426(16):2958-69. doi: 10.1016/j.jmb.2014.06.009.

Structural and enzymatic characterization of a host-specificity determinant from Salmonella. Kohler AC, Spanò S, Galán JE, Stebbins CE. Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):384-91. doi: 10.1107/S1399004713028393.

The inner rod protein controls substrate switching and needle length in a Salmonella type III secretion system. Lefebre MD, Galán JE. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):817-22. doi: 10.1073/pnas.1319698111.

2013

A novel anti-microbial function for a familiar Rab GTPase. Spanò S, Galán JE. Small GTPases. 2013 Oct-Dec;4(4):252-4. doi: 10.4161/sgtp.27282.

Salmonella modulation of host cell gene expression promotes its intracellular growth. Hannemann S, Gao B, Galán JE. PLoS Pathog. 2013;9(10):e1003668. doi: 10.1371/journal.ppat.1003668.

Structure and function of the Salmonella Typhi chimaeric A(2)B(5) typhoid toxin. Song J, Gao X, Galán JE. Nature. 2013 Jul 18;499(7458):350-4. doi: 10.1038/nature12377.

Exploitation of eukaryotic subcellular targeting mechanisms by bacterial effectors. Hicks SW, Galán JE. Nat Rev Microbiol. 2013 May;11(5):316-26. doi: 10.1038/nrmicro3009. Review.

An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA. Chen X, Taylor DW, Fowler CC, Galan JE, Wang HW, Wolin SL. Cell. 2013 Mar 28;153(1):166-77. doi: 10.1016/j.cell.2013.02.037.

Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery. Carleton HA, Lara-Tejero M, Liu X, Galán JE. Nat Commun. 2013;4:1590. doi: 10.1038/ncomms2594.

2012

Contribution of amino acid catabolism to the tissue specific persistence of Campylobacter jejuni in a murine colonization model. Hofreuter D, Mohr J, Wensel O, Rademacher S, Schreiber K, Schomburg D, Gao B, Galán JE. PLoS One. 2012;7(11):e50699. doi: 10.1371/journal.pone.0050699.

A Salmonella Typhi homologue of bacteriophage muramidases controls typhoid toxin secretion. Hodak H, Galán JE. EMBO Rep. 2013 Jan;14(1):95-102. doi: 10.1038/embor.2012.186.

A Rab32-dependent pathway contributes to Salmonella typhi host restriction. Spanò S, Galán JE. Science. 2012 Nov 16;338(6109):960-3. doi: 10.1126/science.1229224.

The Salmonella type III secretion system inner rod protein PrgJ is partially folded. Zhong D, Lefebre M, Kaur K, McDowell MA, Gdowski C, Jo S, Wang Y, Benedict SH, Lea SM, Galan JE, De Guzman RN. J Biol Chem. 2012 Jul 20;287(30):25303-11. doi: 10.1074/jbc.M112.381574.

Quantitative Proteomics of Intracellular Campylobacter jejuni Reveals Metabolic Reprogramming. Liu X, Gao B, Novik V, Galán JE. PLoS Pathog. 2012;8(3):e1002562. doi: 10.1371/journal.ppat.1002562.